7V1M

Structural basis for the co-chaperone relationship of sNASP and ASF1b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9786
Spacegroup name	P 1
Unit cell lengths	71.951, 71.896, 93.427
Unit cell angles	70.67, 70.64, 83.61

Refinement procedure

Resolution	35.588 - 2.834
R-factor	0.2097
Rwork	0.202
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v1l 5bnx
RMSD bond length	0.012
RMSD bond angle	1.245
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.950
High resolution limit [Å]	2.834	6.130	2.850
Rmerge	0.101	0.058	0.752
Rmeas	0.118	0.068	0.890
Rpim	0.060	0.035	0.471
Number of reflections	37598	3786	3563
<I/σ(I)>	4.2
Completeness [%]	95.6	96.4	91.3
Redundancy	3.6	3.7	3.3
CC(1/2)		0.993	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	8% v/v Tacsimate, pH 6.0, 20% w/v PEG 3350