7UZD
Structure of the SARS-CoV-2 RBD in complex with the mouse antibody Fab fragment, HSW-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 123.569, 123.569, 145.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.080 - 3.000 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7sc1 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.080 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.233 | 2.533 |
| Rmeas | 0.238 | 2.586 |
| Rpim | 0.047 | 0.506 |
| Number of reflections | 23278 | 3661 |
| <I/σ(I)> | 12.3 | 3.2 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 26.1 | 26.2 |
| CC(1/2) | 0.998 | 0.354 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium chloride, 0.1 M sodium/potassium phosphate pH 6.5, 25 % PEG 1000 |
