7UYM

850 Fab in complex with NANPNANPNANP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-02-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033167
Spacegroup name	I 41
Unit cell lengths	137.500, 137.500, 63.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.530 - 2.200
R-factor	0.171
Rwork	0.169
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d01
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	XPREP
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	30049	3487
<I/σ(I)>	10.62	1.9
Completeness [%]	99.6	97.3
Redundancy	7.69
CC(1/2)	0.996	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M KCl, 20% (w/v) polyethylene glycol 2250