7UYI
Crystal structure of the computationally optimized broadly reactive H1 influenza hemagglutinin P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 264.849, 77.560, 222.523 |
| Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
| Resolution | 47.750 - 3.000 |
| R-factor | 0.2281 |
| Rwork | 0.226 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.478 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.222 | 1.435 |
| Number of reflections | 90769 | 4343 |
| <I/σ(I)> | 6.6 | 1.7 |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 6.2 | |
| CC(1/2) | 0.988 | 0.429 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.25 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, 15% PEG4000, 6% PEG400 |
