7UYG
Structure of the first OTU domain from Legionella pneumophila effector protein LotA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.245, 86.245, 92.304 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.800 - 1.500 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.22050 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.769 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.070 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.035 | 0.819 |
Number of reflections | 56168 | 2895 |
<I/σ(I)> | 26.6 | 2.2 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG 2K MME, 0.2 M KI, 0.1 M MES pH 6.5 |