7UXZ
Crystal structure of SARS-CoV-2 nucleocapsid protein C-terminal domain complexed with Chicoric acid
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9772 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.669, 120.655, 128.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.980 - 1.733 |
| Rwork | 0.174 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c22 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.628 |
| Data reduction software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.980 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 71415 | 6959 |
| <I/σ(I)> | 9.46 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.995 | 0.509 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 100 mM Tris -HCl (pH 8.3), 30% PEG 4000, 0.2 M sodium acetate |
