7UXZ

Crystal structure of SARS-CoV-2 nucleocapsid protein C-terminal domain complexed with Chicoric acid

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRUS BEAMLINE MANACA
Synchrotron site	LNLS SIRUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9772
Spacegroup name	P 21 21 21
Unit cell lengths	43.669, 120.655, 128.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.980 - 1.733
Rwork	0.174
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c22
RMSD bond length	0.009
RMSD bond angle	1.628
Data reduction software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.980	1.790
High resolution limit [Å]	1.730	1.730
Number of reflections	71415	6959
<I/σ(I)>	9.46
Completeness [%]	99.9
Redundancy	10.6
CC(1/2)	0.995	0.509

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	291	100 mM Tris -HCl (pH 8.3), 30% PEG 4000, 0.2 M sodium acetate