7UXK

Structure of CDK2 in complex with FP24322, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-15
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97861
Spacegroup name	P 41 21 2
Unit cell lengths	65.804, 65.804, 143.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.530 - 2.630
R-factor	0.2628
Rwork	0.261
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kd1
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.530	2.720
High resolution limit [Å]	2.630	2.630
Rmerge	0.129
Number of reflections	9922	938
<I/σ(I)>	16.1
Completeness [%]	99.5
Redundancy	16.1
CC(1/2)	0.999	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Crystallization buffer: 0.1 M BICINE pH 9.2, 2% v/v 1,4-Dioxane, 6% w/v PEG 20000