7UWU

Starch adherence system protein 6 (Sas6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	69.170, 82.370, 213.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.770 - 2.190
R-factor	0.1893
Rwork	0.186
R-free	0.25510
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.851
Data reduction software	xia2
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.770	2.270
High resolution limit [Å]	2.190	2.190
Rmerge	0.090	0.190
Number of reflections	63244	309
<I/σ(I)>	22.7	14.3
Completeness [%]	99.3
Redundancy	16.6
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, potassium thiocyanate, Anderson Evans polyoxotungstate (TEW)