7UW8

Drosophila melanogaster setdb1-tuor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-03
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54178
Spacegroup name	I 2 2 2
Unit cell lengths	59.989, 110.673, 115.313
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.910 - 2.500
R-factor	0.238
Rwork	0.237
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bhd
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.920	39.920	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.080	0.019	1.369
Rmeas	0.086	0.021	1.474
Rpim	0.032	0.008	0.541
Total number of observations	96242	2033	10344
Number of reflections	13531	331	1451
<I/σ(I)>	21.7	83.5	1.9
Completeness [%]	99.4	98.2	97
Redundancy	7.1	6.1	7.1
CC(1/2)	0.999	0.999	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	1.6M ammonium sulphate, 0.01M Magnesium Chloride,0.1M tris pH8.5