7UVM

Crystal structure of human ClpP protease in complex with TR-27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	143.148, 153.722, 105.101
Unit cell angles	90.00, 117.76, 90.00

Refinement procedure

Resolution	29.400 - 2.190
R-factor	0.1979
Rwork	0.197
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bba
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.620	2.250
High resolution limit [Å]	2.190	2.190
Number of reflections	102300	9495
<I/σ(I)>	5.2
Completeness [%]	99.2
Redundancy	7
CC(1/2)	0.987	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M sodium acetate pH 4.6 to 5.2, 5 % PEG 4000