7UVF

Crystal structure of ZED8 Fab complex with CD8 alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2015-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	C 1 2 1
Unit cell lengths	268.968, 59.441, 111.377
Unit cell angles	90.00, 112.29, 90.00

Refinement procedure

Resolution	34.355 - 2.600
R-factor	0.2008
Rwork	0.198
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Internal
RMSD bond length	0.008
RMSD bond angle	1.014
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.076	0.026	0.838
Rmeas	0.089	0.032
Rpim	0.050	0.017	0.675
Total number of observations	151360
Number of reflections	48140	2587	1947
<I/σ(I)>	8.4
Completeness [%]	94.7	96.7	78.4
Redundancy	3.1	3.4	1.9
CC(1/2)		0.999	0.442

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	292	0.1M ammonium acetate 0.1M Bis-Tris pH6.0 17% PEG 10K