7UVF
Crystal structure of ZED8 Fab complex with CD8 alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 268.968, 59.441, 111.377 |
| Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
| Resolution | 34.355 - 2.600 |
| R-factor | 0.2008 |
| Rwork | 0.198 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Internal |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.014 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.076 | 0.026 | 0.838 |
| Rmeas | 0.089 | 0.032 | |
| Rpim | 0.050 | 0.017 | 0.675 |
| Total number of observations | 151360 | ||
| Number of reflections | 48140 | 2587 | 1947 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 94.7 | 96.7 | 78.4 |
| Redundancy | 3.1 | 3.4 | 1.9 |
| CC(1/2) | 0.999 | 0.442 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 292 | 0.1M ammonium acetate 0.1M Bis-Tris pH6.0 17% PEG 10K |
