7UUN

Crystal structure of aminoglycoside resistance enzyme ApmA, complex with neomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97919
Spacegroup name	P 43 3 2
Unit cell lengths	133.564, 133.564, 133.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.800 - 2.830
R-factor	0.2133
Rwork	0.211
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm2
RMSD bond length	0.005
RMSD bond angle	0.885
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.880
High resolution limit [Å]	2.830	2.830
Rmerge	0.102	4.877
Rpim	0.016	0.770
Number of reflections	10203	492
<I/σ(I)>	54.3	1.1
Completeness [%]	99.9	100
Redundancy	40.9
CC(1/2)	1.000	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2 M lithium sulfate, 0.1 M Tris pH 8.5, 25% PEG3350, 10 mM neomycin