7USJ

BRD4-BD2 in complex with SF2523

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2022-02-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	77.625, 77.625, 100.645
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.820 - 2.080
R-factor	0.2187
Rwork	0.214
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uf0
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.820	2.154
High resolution limit [Å]	2.080	2.080
Rmerge	0.192
Rpim	0.055
Number of reflections	19034	1873
<I/σ(I)>	15.1
Completeness [%]	99.5
Redundancy	10.6
CC(1/2)	0.999	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289.15	0.1 M HEPES pH 7.5, 28 % w/v PEG 3350