7USI

BRD2-BD1 in complex with MDP5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	63.810, 149.872, 63.819
Unit cell angles	90.00, 112.27, 90.00

Refinement procedure

Resolution	35.560 - 2.500
R-factor	0.2035
Rwork	0.201
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jke
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.560	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.126
Rpim	0.048
Number of reflections	38336	3871
<I/σ(I)>	24.6
Completeness [%]	99.7
Redundancy	6.9
CC(1/2)	0.978	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289.15	0.2 M Sodium tartrate dibasic dihydrate pH 7.5 and 20 % w/v PEG 1000