7US1

Structure of parkin (R0RB) bound to two phospho-ubiquitin molecules

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1808
Spacegroup name	P 61 2 2
Unit cell lengths	83.078, 83.078, 253.816
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.590 - 2.484
R-factor	0.2309
Rwork	0.229
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6glc
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.590	2.573
High resolution limit [Å]	2.484	2.484
Number of reflections	19185	1847
<I/σ(I)>	21.08
Completeness [%]	99.9
Redundancy	18.3
CC(1/2)	0.999	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	0.1M Bis-Tris propane pH 7.5, 20.5% PEG3350, 0.2M NaI