7URK
Self-assembling DNA tensegrity triangle motif with intercalating internal Cy3 modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-22 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.18076 |
| Spacegroup name | H 3 |
| Unit cell lengths | 113.785, 113.785, 95.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.330 - 3.120 |
| R-factor | 0.1551 |
| Rwork | 0.155 |
| R-free | 0.15660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.590 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER (1.18.2_3874) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.330 | 34.328 | 3.439 |
| High resolution limit [Å] | 3.119 | 10.101 | 3.119 |
| Number of reflections | 4656 | 230 | 233 |
| <I/σ(I)> | 12.5 | 46.4 | 2.9 |
| Completeness [%] | 75.0 | ||
| Redundancy | 9.9 | ||
| CC(1/2) | 1.000 | 1.000 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 120 mM Tris, 583 mM ammonium sulfate, 37.5 mM magnesium acetate, 60 mM acetic acid, 6 mM EDTA |
