7UR0
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold-insert duplexes (21mer and 10mer) containing the cognate REPE54 sequence and an additional T-A rich sequence, respectively.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2021-06-30 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 74.407, 127.493, 135.478 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.610 - 3.300 |
R-factor | 0.2331 |
Rwork | 0.228 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7u6k |
RMSD bond length | 0.004 |
RMSD bond angle | 0.775 |
Data reduction software | XDS (Jan 31, 2020) |
Data scaling software | XSCALE (Jan 31, 2020) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.610 | 3.418 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.385 | 1.218 |
Rmeas | 0.417 | 1.314 |
Rpim | 0.160 | 0.490 |
Number of reflections | 10028 | 967 |
<I/σ(I)> | 5.91 | 2.58 |
Completeness [%] | 97.8 | 96.94 |
Redundancy | 6.9 | 7.1 |
CC(1/2) | 0.990 | 0.904 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 120 mM MgCl2, 20% PEG 400, 100 mM Tris HCl pH 8.0 |