7UQL

Pathogenesis related 10-10 app from

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	70.175, 92.547, 52.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.090 - 1.900
R-factor	0.2104
Rwork	0.207
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ka3
RMSD bond length	0.010
RMSD bond angle	0.947
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600	1.950
High resolution limit [Å]	1.900	1.900
Number of reflections	27479	1977
<I/σ(I)>	27.42
Completeness [%]	98.5
Redundancy	8.1
CC(1/2)	1.000	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Bis-Tris pH 5.5