7UQ2

Vs.4 from T4 phage in complex with cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0448
Spacegroup name	P 41 21 2
Unit cell lengths	81.694, 81.694, 217.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.850 - 2.000
R-factor	0.1836
Rwork	0.183
R-free	0.20610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold model
RMSD bond length	0.015
RMSD bond angle	1.229
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmeas	0.101
Rpim	0.026	0.346
Number of reflections	49941	2436
<I/σ(I)>	33.6	2.17
Completeness [%]	98.2
Redundancy	14.4
CC(1/2)	0.984	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	7% PEG8000, 0.1 M imidazole, pH 6.3, 0.2 mM calcium acetate