7UPS

Structural study of Legionella pneumophila effector DotY (Lpg0294)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97935
Spacegroup name	C 1 2 1
Unit cell lengths	153.200, 75.620, 101.200
Unit cell angles	90.00, 127.91, 90.00

Refinement procedure

Resolution	43.540 - 2.430
R-factor	0.2379
Rwork	0.237
R-free	0.26220
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.567
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.550	2.550
High resolution limit [Å]	2.430	2.430
Number of reflections	33201	3535
<I/σ(I)>	9.35
Completeness [%]	95.5
Redundancy	6.4
CC(1/2)	0.998	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Sodium Citrate pH3.5, 25% PEG8k and 0.3M Sodium Acetate