7UN7

DNA Polymerase lambda in complex with a 1nt microhomology substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.135, 62.774, 140.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.760 - 2.040
R-factor	0.2212
Rwork	0.220
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.113	52.113	2.089
High resolution limit [Å]	2.040	5.845	2.040
Rmerge	0.200	0.072	9.900
Rmeas	0.385	0.075	10.876
Rpim	0.106	0.022	2.986
Number of reflections	30399	1519	1522
<I/σ(I)>	10.6
Completeness [%]	97.3	100	98.6
Redundancy	13.3	12.9	13.8
CC(1/2)	0.932	0.901	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG