7UMI
Importin a1 bound to Cp183-CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.436, 90.688, 97.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.090 - 1.990 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7leu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.945 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rpim | 0.033 | 0.605 |
| Number of reflections | 47594 | 47594 |
| <I/σ(I)> | 11.5 | 1.5 |
| Completeness [%] | 98.8 | 99.3 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.6 M sodium citrate, 0.1 M HEPES pH 7.2, 10 mM DTT |
