7UM3
Crystal structure of a Fab in complex with a peptide derived from the LAG-3 D1 domain loop insertion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.238, 112.620, 125.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.855 - 2.398 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nm4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.855 | 2.460 |
| High resolution limit [Å] | 2.398 | 2.400 |
| Number of reflections | 36288 | 2480 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.860 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Bis-Tris, pH 5.5, 25% PEG3350 |
