7ULN

Turnip yellows virus N-terminal readthrough domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	46.434, 74.859, 130.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.389 - 1.530
R-factor	0.1751
Rwork	0.174
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ulo
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	130.780	130.780	1.550
High resolution limit [Å]	1.530	8.360	1.530
Rmerge	0.070	0.041	0.729
Rmeas	0.076	0.045	0.794
Rpim	0.029	0.017	0.310
Total number of observations		3062	20257
Number of reflections	69780	514	3206
<I/σ(I)>	20.5	62.3	2.2
Completeness [%]	99.5	99.1	93.1
Redundancy	6.7	6	6.3
CC(1/2)	0.999	0.999	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	293.15	0.1 M HEPES, pH 7.7, 1.4 M ammonium sulfate, 0.1 M sodium chloride