Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-25 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.143, 91.423, 91.922 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.560 |
| R-factor | 0.2342 |
| Rwork | 0.232 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vzk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.600 |
| High resolution limit [Å] | 2.560 | 6.940 | 2.560 |
| Rmerge | 0.232 | 0.120 | 0.812 |
| Rmeas | 0.253 | 0.133 | 0.897 |
| Rpim | 0.099 | 0.056 | 0.377 |
| Total number of observations | 130243 | ||
| Number of reflections | 20515 | 1139 | 1018 |
| <I/σ(I)> | 5 | ||
| Completeness [%] | 100.0 | 99.6 | 99.8 |
| Redundancy | 6.3 | 5.5 | 5.4 |
| CC(1/2) | 0.985 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 0.1 M Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, 0.1 M Ammonium sulfate, 25% PEG 3350, 19mM Methyl 6-O-(N-heptylcarbamoyl)-alpha-D-glucopyranoside |
