7UGR

Crystal structure of hyperfolder YFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	51.994, 66.872, 143.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.393 - 1.740
R-factor	0.2033
Rwork	0.202
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wj2
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.393	1.802
High resolution limit [Å]	1.740	1.740
Rmerge	0.085	0.325
Rmeas	0.103	0.392
Rpim	0.057	0.216
Number of reflections	46199	2564
<I/σ(I)>	8.58	2.46
Completeness [%]	97.4	99.77
Redundancy	3.1	3.2
CC(1/2)	0.989	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	0.1 M Tris-HCl pH 8.5, 25% PEG 3350, 0.2 M sodium acetate