7UGK
Crystal structure of the human queuine salvage enzyme DUF2419, wild-type apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.689, 137.513, 52.804 |
| Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
| Resolution | 49.340 - 1.780 |
| R-factor | 0.1332 |
| Rwork | 0.132 |
| R-free | 0.16030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7u07 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.354 |
| Data reduction software | HKL-3000 (v721.3) |
| Data scaling software | HKL-3000 (v721.3) |
| Phasing software | PHASER (phenix v1.19.2) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
| Rmerge | 0.086 | 0.035 | 0.558 |
| Rmeas | 0.094 | 0.038 | 0.612 |
| Rpim | 0.036 | 0.014 | 0.245 |
| Total number of observations | 371082 | ||
| Number of reflections | 56376 | 2909 | 2519 |
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 96.8 | 98.2 | 88.1 |
| Redundancy | 6.6 | 6.9 | 6 |
| CC(1/2) | 0.999 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 0.2M NaCl, 25% PEG3350, 0.1M Bis-Tris, pH 5.5 |
