7UFN

Structure of PfCSP peptide 21 with antibody P3-21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	36.694, 124.033, 103.635
Unit cell angles	90.00, 90.13, 90.00

Refinement procedure

Resolution	51.820 - 2.200
R-factor	0.1662
Rwork	0.164
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b5m
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.820	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	46287	4700
<I/σ(I)>	5.74
Completeness [%]	99.8
Redundancy	3.6
CC(1/2)	0.980	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	5% isopropanol, 2 M Lithium sulfate, 0.1 M Magnesium sulfate, and 0.1 M sodium acetate trihydrate pH 4.5