7UDK

Crystal structure of designed helical repeat protein RPB_LRP2_R4 bound to LRPx4 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0329
Spacegroup name	P 32 2 1
Unit cell lengths	70.780, 70.780, 128.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.390 - 3.180
R-factor	0.2096
Rwork	0.207
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted model
RMSD bond length	0.004
RMSD bond angle	0.616
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.390	3.294
High resolution limit [Å]	3.180	3.180
Rmerge	0.080
Rmeas	0.085
Rpim	0.027
Number of reflections	6621	633
<I/σ(I)>	14.13
Completeness [%]	89.0
Redundancy	9.8
CC(1/2)	1.000	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M HEPES, pH 7, 10% w/v PEG6000