7UDA
Structure of the EstG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2021-05-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 |
| Unit cell lengths | 110.321, 110.321, 55.918 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 2.470 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ci8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.620 | 29.600 | 2.570 |
| High resolution limit [Å] | 2.470 | 8.920 | 2.470 |
| Rmerge | 0.108 | 0.047 | 0.781 |
| Rmeas | 0.117 | 0.052 | 0.847 |
| Rpim | 0.045 | 0.020 | 0.324 |
| Total number of observations | 3610 | 16345 | |
| Number of reflections | 24085 | 554 | 2515 |
| <I/σ(I)> | 10.9 | 26.3 | 2.4 |
| Completeness [%] | 99.0 | 97.4 | 91.9 |
| Redundancy | 6.7 | 6.5 | 6.5 |
| CC(1/2) | 0.996 | 0.995 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 20% PEG500 MME, 10% PEG20000, 0.1 M Tris/Bicine pH 8.5 and 90 mM mixture of sodium nitrate, sodium phosphate dibasic and ammonium sulfate |
