7UCX
LRP8 11H1 Fab complexed to a cyclized CR1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.265, 79.827, 67.066 |
| Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
| Resolution | 24.780 - 1.720 |
| R-factor | 0.2067 |
| Rwork | 0.205 |
| R-free | 0.23970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vpo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.004 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 45560 | 6618 |
| <I/σ(I)> | 19.9 | 2.4 |
| Completeness [%] | 99.3 | |
| Redundancy | 3.4 | |
| CC(1/2) | 1.000 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 296 | 25% PEG 4000, 0.2M Ammonium Sulfate, 0.1M Sodium acetate/HCl |
