7UCT
The Crystal Structure of Apo Domain-Swapped Dimer F57:H:H:H:H:H:H:R58 Mutant of HCRBPII with Histidine Insertion in the Hinge Loop Region at 2.5 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-03-20 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97633 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.797, 76.582, 110.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.290 - 2.520 |
| R-factor | 0.1826 |
| Rwork | 0.176 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.290 | 2.580 |
| High resolution limit [Å] | 2.520 | 2.540 |
| Rmerge | 0.090 | 0.458 |
| Rmeas | 0.122 | 0.541 |
| Number of reflections | 10638 | 947 |
| <I/σ(I)> | 13.76 | 2.07 |
| Completeness [%] | 99.2 | 98.5 |
| Redundancy | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, sodium acetate, ammonium acetate |
