7UCF

Structure of the BG505 SOSIP.664 trimer in complex with neutralizing antibody Fab fragments 10-1074 and BG24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	.987
Spacegroup name	H 3
Unit cell lengths	208.958, 208.958, 155.914
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.490 - 4.000
R-factor	0.2472
Rwork	0.245
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez
RMSD bond length	0.017
RMSD bond angle	1.984
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.490	4.060
High resolution limit [Å]	3.800	3.800
Rmerge	0.143	7.290
Rpim	0.066	2.360
Number of reflections	24941	4480
<I/σ(I)>	7.4	0.3
Completeness [%]	99.6
Redundancy	11.3
CC(1/2)	0.998	0.210

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1 M Bis-Tris pH 6.5, 20% PEG1500