7UCF
Structure of the BG505 SOSIP.664 trimer in complex with neutralizing antibody Fab fragments 10-1074 and BG24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .987 |
| Spacegroup name | H 3 |
| Unit cell lengths | 208.958, 208.958, 155.914 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.490 - 4.000 |
| R-factor | 0.2472 |
| Rwork | 0.245 |
| R-free | 0.27720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cez |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.984 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.490 | 4.060 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.143 | 7.290 |
| Rpim | 0.066 | 2.360 |
| Number of reflections | 24941 | 4480 |
| <I/σ(I)> | 7.4 | 0.3 |
| Completeness [%] | 99.6 | |
| Redundancy | 11.3 | |
| CC(1/2) | 0.998 | 0.210 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M Bis-Tris pH 6.5, 20% PEG1500 |
