7UC7
Stat5a Core in complex with Compound 17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-03-24 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9787 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 238.895, 238.895, 113.304 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.880 - 3.102 |
| R-factor | 0.2248 |
| Rwork | 0.222 |
| R-free | 0.28140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | previously solved structure in lab |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.076 | 0.035 | 1.515 |
| Rmeas | 0.079 | 0.037 | 1.586 |
| Rpim | 0.024 | 0.012 | 0.466 |
| Total number of observations | 256562 | ||
| Number of reflections | 22460 | 1168 | 1101 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 99.9 | 98.8 | 100 |
| Redundancy | 11.4 | 10.3 | 11.6 |
| CC(1/2) | 0.997 | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.1M Ammonium Tartrate dibasic pH 7.0 |
