7UBA
Structure of fungal Hop1 CBR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.283 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.389, 38.917, 68.981 |
| Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
| Resolution | 32.600 - 1.599 |
| R-factor | 0.1451 |
| Rwork | 0.144 |
| R-free | 0.16350 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 1.610 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmeas | 0.057 | 0.804 |
| Number of reflections | 65701 | 7028 |
| <I/σ(I)> | 13.93 | |
| Completeness [%] | 98.5 | 61 |
| Redundancy | 3.34 | 1.97 |
| CC(1/2) | 0.999 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH 6.5, 30% PEG 400 |
