7U9H

Crystal Structure of Escherichia coli apo Pyridoxal 5'-phosphate homeostasis protein (YGGS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-02
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.5406
Spacegroup name	I 41 2 2
Unit cell lengths	124.160, 124.160, 82.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.503 - 2.000
R-factor	0.1881
Rwork	0.186
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w8g
Data reduction software	CrysalisPro (41.64.69a)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.503	28.500	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.068	0.016	0.853
Rmeas	0.071	0.016	0.885
Rpim	0.018	0.004	0.235
Total number of observations		3739	22567
Number of reflections	22173	282	1608
<I/σ(I)>	33	107.7	4.2
Completeness [%]	100.0	97	100
Redundancy	15	13.3	14
CC(1/2)	1.000	1.000	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2M Ammonium sulfate, 5% Isopropanol