7U9A
EGFR in complex with a macrocyclic inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 145.825, 145.825, 145.825 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.110 - 2.600 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vhp |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 103.110 | 103.190 | 2.650 |
| High resolution limit [Å] | 2.600 | 7.060 | 2.600 |
| Rmeas | 0.125 | 0.058 | 1.391 |
| Rpim | 0.055 | 0.026 | 0.606 |
| Total number of observations | 80669 | 4015 | 3989 |
| Number of reflections | 16002 | 855 | 778 |
| <I/σ(I)> | 10.4 | 29.4 | 1 |
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 5 | 4.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 1.1 M Sodium Citrate |
