7U8T
Vps13 adaptor binding domain in complex with Mcp1 PxP motif peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.735, 91.225, 132.769 |
| Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
| Resolution | 48.360 - 3.000 |
| R-factor | 0.2514 |
| Rwork | 0.250 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold2 model |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20rc3_4411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.360 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.044 | 0.322 |
| Rmeas | 0.052 | 0.378 |
| Rpim | 0.028 | 0.197 |
| Number of reflections | 39383 | 3799 |
| <I/σ(I)> | 17.2 | 3.4 |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.999 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 295 | 75 mM imidazole pH 8.2, 70 mM lithium sulfate, 500 mM sodium chloride |
