7U8M

Crystal structure of chimeric hemagglutinin cH15/3 in complex with broad protective antibodies 31.a.83 and FluA-20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0331
Spacegroup name	C 1 2 1
Unit cell lengths	272.922, 155.729, 156.893
Unit cell angles	90.00, 94.63, 90.00

Refinement procedure

Resolution	49.940 - 5.390
R-factor	0.306
Rwork	0.304
R-free	0.34200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tg8 4we8 5kaq 6ocb
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.940	5.590
High resolution limit [Å]	5.390	5.390
Rpim	0.560	0.420
Number of reflections	21811	1941
<I/σ(I)>	8.9	1
Completeness [%]	96.3	86.4
Redundancy	3.4	2.4
CC(1/2)	0.990	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium cacodylate, pH 6.5, 0.2 LiSO4, and 30% (w/v) polyethylene glycol 400