7U8L
Crystal structure of chimeric hemagglutinin cH15/3 in complex with broad protective antibody 31.a.83
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 156.168, 156.168, 114.019 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.640 | 4.680 |
High resolution limit [Å] | 4.560 | 4.560 |
Rpim | 0.060 | 0.370 |
Number of reflections | 9360 | 431 |
<I/σ(I)> | 7.5 | 0.9 |
Completeness [%] | 99.5 | 95.4 |
Redundancy | 12.3 | 4.2 |
CC(1/2) | 0.994 | 0.555 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris, pH 7.0, 40% (w/v) polyethylene glycol 300 and 5% (w/v) polyethylene glycol 1000 |