7U8C

Crystal structure of Mesothelin C-terminal peptide-MORAb 15B6 FAB complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03317
Spacegroup name	P 43 21 2
Unit cell lengths	64.832, 64.832, 250.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.010 - 1.740
R-factor	0.2061
Rwork	0.204
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rif
RMSD bond length	0.003
RMSD bond angle	0.534
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.740	1.740
Rmerge	0.092	0.497
Number of reflections	53658	3899
<I/σ(I)>	35
Completeness [%]	95.3
Redundancy	27.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M sodium acetate, pH 4.5, 26% PEG550 MME, 6% 2-propanol