7U8B

Product of 14mer primer with activated asymmetric GA dimer diastereomer 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	99
Detector technology	PIXEL
Collection date	2022-02-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033167
Spacegroup name	P 3 2 1
Unit cell lengths	43.455, 43.455, 81.122
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.162 - 1.730
Rwork	0.194
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8o
RMSD bond length	0.023
RMSD bond angle	2.965
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.093	0.499
Rmeas	0.103	0.554
Rpim	0.043	0.230
Number of reflections	9404	474
<I/σ(I)>	13.8	4.8
Completeness [%]	96.3	98.5
Redundancy	5	4.8
CC(1/2)	0.998	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	1.7 M Lithium sulfate, 50 mM HEPES pH 7.0, 50 mM Magnesium sulfate