7U88

Product of 13mer primer with activated G monomer diastereomer 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	98
Detector technology	CCD
Collection date	2021-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 3 2 1
Unit cell lengths	42.723, 42.723, 85.822
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.948 - 2.140
Rwork	0.214
R-free	0.29160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8o
RMSD bond length	0.018
RMSD bond angle	2.603
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.140	2.140
Rmerge	0.077	0.402
Rmeas	0.082	0.438
Rpim	0.027	0.166
Number of reflections	5344	229
<I/σ(I)>	24.2	2.2
Completeness [%]	99.2
Redundancy	9.5	5.7
CC(1/2)	1.000	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	1.5 M Lithium sulfate, 50 mM TRIS pH 8.5, 5% w/v Glycerol