7U87

Product of 13mer primer with activated G monomer diastereomer 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	98
Detector technology	CCD
Collection date	2021-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 3 2 1
Unit cell lengths	42.741, 42.741, 83.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.675 - 1.701
Rwork	0.188
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8o
RMSD bond length	0.033
RMSD bond angle	3.256
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.089	0.348
Rmeas	0.094	0.376
Rpim	0.030	0.139
Number of reflections	10199	474
<I/σ(I)>	19.4	2.7
Completeness [%]	99.9
Redundancy	9.9	6.5
CC(1/2)	1.000	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	0.2 M Ammonium acetate, 0.1 M BIS-TRIS pH 6.5, 45% v/v (+/-)-2-Methyl-2,4-pentanediol