7U7Z

Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 0.12 mM Mn2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-03-31
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.215, 98.215, 82.157
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.770 - 1.670
R-factor	0.1888
Rwork	0.186
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.770	37.770	1.770
High resolution limit [Å]	1.670	4.970	1.670
Rmerge	0.057	0.020	1.371
Rmeas	0.062	0.022	1.516
Total number of observations	589069
Number of reflections	103090	3880	16489
<I/σ(I)>	16.29	61.26	1.11
Completeness [%]	99.8	99.7	98.8
Redundancy	5.714	5.703	5.491
CC(1/2)	1.000	1.000	0.453

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES