7U7W

Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 1.0 mM Mg2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-02-06
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	97.969, 97.969, 81.761
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.420 - 1.660
R-factor	0.1851
Rwork	0.184
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.420	42.420	1.760
High resolution limit [Å]	1.660	4.960	1.660
Rmerge	0.050	0.020	1.048
Rmeas	0.055	0.022	1.154
Total number of observations	588160
Number of reflections	101079	3860	15946
<I/σ(I)>	18.85	66.43	1.46
Completeness [%]	97.8	99.3	95.4
Redundancy	5.819	5.725	5.634
CC(1/2)	1.000	1.000	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES