7U7F

Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 10.0 mM Mn2+ for 90s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-12-14
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 61
Unit cell lengths	98.269, 98.269, 81.983
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.550 - 1.650
R-factor	0.2035
Rwork	0.200
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kfh
RMSD bond length	0.009
RMSD bond angle	1.195
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.550	42.550	1.750
High resolution limit [Å]	1.650	4.920	1.650
Rmerge	0.058	0.023	1.312
Rmeas	0.064	0.025	1.450
Total number of observations	591871
Number of reflections	104851	3986	17059
<I/σ(I)>	15.75	58.61	1.13
Completeness [%]	98.7	99.8	99.1
Redundancy	5.645	5.645	5.484
CC(1/2)	0.999	0.999	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES