7U5P

CRYSTAL STRUCTURE OF THE ACTIVIN RECEPTOR TYPE-2A LIGAND BINDING DOMAIN IN COMPLEX WITH ACTIVIN-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-22
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	82.610, 82.540, 151.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.690 - 3.140
R-factor	0.2232
Rwork	0.217
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s4y
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.690	3.250
High resolution limit [Å]	3.140	3.140
Rmerge	0.065	0.473
Number of reflections	17787	1631
<I/σ(I)>	2.949
Completeness [%]	95.4
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	294	100mM Sodium Citrate, 17%PEG 3000