7U4S

Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	73.718, 126.899, 86.820
Unit cell angles	90.00, 100.32, 90.00

Refinement procedure

Resolution	72.520 - 2.680
R-factor	0.2388
Rwork	0.236
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iq8
RMSD bond length	0.001
RMSD bond angle	0.427
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.520	2.750
High resolution limit [Å]	2.680	2.680
Rpim	0.183	2.205
Number of reflections	44083	3258
<I/σ(I)>	4.7
Completeness [%]	100.0	100
Redundancy	13	7
CC(1/2)	0.966	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	20% w/v PEG6000 0.1 M citrate, pH 4.0, 1.0 M lithium chloride