7U4P
Covalently stabilized triangular trimer composed of Abeta17-36 beta-hairpins
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 113 |
| Detector technology | PIXEL |
| Collection date | 2019-12-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 62.054, 62.054, 47.839 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.030 - 1.803 |
| R-factor | 0.2092 |
| Rwork | 0.205 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7jxo |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.030 | 1.868 |
| High resolution limit [Å] | 1.803 | 1.803 |
| Rmerge | 0.064 | 0.930 |
| Number of reflections | 5373 | 522 |
| <I/σ(I)> | 39.93 | |
| Completeness [%] | 99.7 | |
| Redundancy | 36.2 | |
| CC(1/2) | 1.000 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1 M Tris at pH 8.3, 0.2 M MgCl2, 2.8 M 1,6-hexanediol |
